High viability bench-top stem cell sorter – Introducing the WOLF G2

Looking for high viability bench-top stem cell sorting? Meet the WOLF G2 cell sorter. The gold standard for cell sorting of fragile stem cells to give high cell viability. The WOLF G2 Cell Sorter was originally created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to effectively and easily sort high-quality cells. Now, as science continues to move forward into increasingly complex realms, the WOLF is moving forward with it. The WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single- cell dispensing. Single-cell sorting can be completed in 96- or 384-well plates when using the WOLF G2 in conjunction with the N1 Single-Cell Dispenser. This flexibility in performance, along with the additional abilities of the second laser makes it ideal for use in many different research fields and application areas like single-cell genomics, cell line development, gene editing, antibody discovery, immunology, infectious disease, basic research, and more.

The WOLF G2 is a high viability bench-top stem cell sorter giving you:

+ Healthy Cells at < 2 psi, the WOLF G2 are gentler than any conventional cell sorters, enabling healthier cells post-sort, especially for engineered lines, primary cells, and stem cells.

+ Contaminant- and Biohazard-Free. Disposable, aerosol-free microfluidic cartridge isolates the sample from the environment, while protecting the user from exposure.

+ Simple to use. Intuitive software, fixed optics, no fluidics cart and less than one minute clean-up time.

+ High Sensitivity and Resolution. All laser configurations afford < 250 MESF sensitivity, along with forward and back scatter, providing as low as 1 µm resolution.

+ Compact. At under 2 cubic feet, NanoCellect’s benchmark for access and performance allows every lab for the flexibility to do analysis and sorting into tubes or 96- and 384-well plates.

Expanding the WOLF’s Capabilities. With two lasers and up to nine fluorescent channels, the WOLF G2 aligns with a broad set of research applications and experiments. Three different laser configurations allow options specific to your needs.

There are various applications for WOLF G2 cell sorter including:

Gene Editing. Gentle microfluidic cell sorting with the WOLF G2 uses pressures of below 2psi. The combined power of the WOLF G2 and the N1 Single-Cell Dispenser eliminates the main challenges typically facing pluripotent stem cell processing. Without decompression shock and shear stress, single-cell deposition results in high monoclonal outgrowth. This improved workflow will allow for higher throughput pluripotent stem cell research, which is valuable for both basic and clinical research.

Infectious Disease. The WOLF G2 microfluidic cell sorter is aerosol-free and compact enough to fit into standard biosafety hoods, making it ideal for infectious disease research. The WOLF G2’s disposable cartridges also avoid cross-contamination and create a safe and effective workflow for the kind of speed and efficiency required to combat infectious disease threats

Immunology and Antibody Discovery. The WOLF G2 Cell Sorter provides a simple cell sorting solution that gently sorts high antibody-producing clones and dispenses single cells for optimal clone outgrowth. With two lasers and up to nine fluorescent channels, the WOLF G2 provides the most comprehensive benchtop cell sorting solution

Cell Line Development. The WOLF G2 Cell Sorter protects cell viability after sorting and improves sorting efficiency by increasing the number of viable clones growing per plate.. Contaminant- and biohazard–free, the WOLF G2 Cell Sorter’s rapidly exchangeable cartridge and tubing set eliminates carryover between samples and allows for quick and easy cleanup. When paired with the N1 Single-Cell Dispenser, the WOLF G2 can select viable single cells and plate them into 96- or 384-well plates. Low pressure microfluidics greatly improves cell integrity over conventional droplet sorters, resulting in high-outgrowth and high-titer monoclonal lines. Blood Filter Sample Prep Single-Cell NGS

Genomics. The WOLF G2’s gentle microfluidic system eliminates unintentional sequencing and analysis of dying or off-target cells or debris while maintaining cell integrity and avoiding stressing cells and affecting downstream data. Researchers can sort cells with a wide range of genome sizes, including human, plant, and animal cells or microorganisms like yeast and bacteria, providing insight on population genetics or disease. The WOLF G2 also allows labs to be completely flexible when it comes to sequencing research and eliminates carryover with a 100% disposable fluidic cartridge.

Microfluidic Cartridge Technology

The WOLF G2 Cell Sorter uses patented, microfluidic-based sorting with robust laser-excitation and sensitive PMT detectors to isolate mammalian cells, microbes, plant cells and more. A gentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup.

The high viability bench-top stem cell sorter – WOLF G2 boasts:

Monoclonal HEK Outgrowth. The same 25% GFP+RFP+ dual-expressor cell mix was also sorted for single cells into 96-well plates and incubated for 14 days. As a rigorous limiting dilution comparison, 100% GFP+RFP+ dual-expressor cells were dispensed at 1 cell/well. The WOLF G2 with N1 plate still yielded more than a 2-fold increase in targeted monoclonal colonie

N1 Single-Cell Dispenser. Designed to sort and dispense into 96- and 384- well plates, the N1 provides higher rates of singlet detection compared to cell printers or limiting dilution. Users can perform simple, label-free dispensing or advanced multicolor panel single-cell dispensing.

WOLFViewer Software. The WOLFViewer software has an intuitive workflow menu that walks users through their experimental process, and is designed for both novice and expert users. New users can be performing their first sorts in about 20 minutes and can be on their own on day one.

So what are you waiting for! The WOLF G2 is the cell sorter offering High cell integrity without compromise

Healthy Cells

Broad Capabilities

Better Science

Blog by Amina Surve. Swift Analytical Ltd. Suppliers of the WOLF G2 cell sorter. email: amina.surve@swiftanalytical.com